INTRODUCTION: Coronary artery disease (CAD) is one of the major causes of death in the world and is based on both environmental and genetic factors. Single nucleotide polymorphism (SNP) is a variation of one nucleotide sequence at any region of genome that may cause disease susceptibility. Therefore, SNPs have been proposed as ideal markers in disease association studies. Endothelial lipase (EL) is a protein from triglyceride lipase family that plays an important role in high density lipoprotein (HDL) metabolism. The EL gene has a common 584 C/T polymorphism, but it is unclear whether this polymorphism is associated with HDL-cholesterol levels or CAD. So, the purpose of this study was to investigate the relationship between the EL 584C/T gene polymorphism, HDL levels and risk of CAD in Elazıg province.
METHODS: The population of this study consists of 78 patients with angiographically confirmed CAD and 81 healthy controls. Genotyping for 584 C/T polymorphism was detected with the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. Data were analyzed using SPSS.
RESULTS: The frequencies of the CT, CC, and TT genotypes were 40.74, 56.8, and 2.46 %, respectively, within the control group, and 66.67, 30.37, and 2.56%, respectively, in the CAD group. Hardy-Weinberg values are p<0.05 for control, p>0.05 for CAD group. No significant association was found between the 584 C/T variant and HDL-C levels. T allele frequencies were higher in CAD group than control.
DISCUSSION AND CONCLUSION: It was concluded that the T allele was associated with risk of CAD in xxxxxxxxx province independent of HDL-cholesterol level.