ISSN  2587-2362  |  E-ISSN  2618-642X
Examination of fasting and postprandial dynamic thiol disulfide homeostasis and oxidative stress [Int J Med Biochem ]
Int J Med Biochem . 2021; 4(3): 185-191 | DOI: 10.14744/ijmb.2021.53825

Examination of fasting and postprandial dynamic thiol disulfide homeostasis and oxidative stress

Ahmet Rıfat Balık1, Betül Özbek İpteç1, Reyhan Ersoy2, Gulsen Yilmaz1, Ozcan Erel1, Cemile Biçer1
1Department of Medical Biochemistry, Health Sciences University Gulhane Training and Research Hospital, Ankara, Turkey
2Department of Medical Biochemistry, Ankara Yildirim Beyazit University, Faculty of Medicine, Ankara, Turkey
3Department of Endocrinology, Ankara Yildirim Beyazit University, Faculty of Medicine, Ankara, Turkey

INTRODUCTION: Oxidative stress has a central role in metabolic disorders associated with high-carbohydrate, animal-based-protein diets, and excessive fat consumption. However, the molecular mechanisms of nutrition-mediated oxidative stress are complex and not fully understood. Dynamic thiol-disulfide homeostasis (DTDH) is the regulation of a balance between thiols and their oxidized forms, and includes the reversal of thiol oxidation in proteins. This study was an evaluation of DTDH and postprandial oxidative stress.
METHODS: A total of 86 participants (43 males and 43 females), were included in the study. Fasting and non-fasting blood samples were collected and the native thiol, total thiol, and disulfide parameters were analyzed. Statistical analyses were performed using IBM SPSS Statistics for Windows, Version 22.0 (IBM Corp., Armonk, NY, USA).
RESULTS: The findings indicated that while the native thiol values were significantly lower in the postprandial samples, the disulfide values were significantly higher. There was no significant difference in the total thiol values.
DISCUSSION AND CONCLUSION: Examination of the DTDH revealed that the oxidative stress level increased following food intake. Protein thiols involved in antioxidant defense were oxidized and transformed into disulfides.

Keywords: Fasting, non-fasting, postprandial oxidative stress, thiol-disulfide.

Ahmet Rıfat Balık, Betül Özbek İpteç, Reyhan Ersoy, Gulsen Yilmaz, Ozcan Erel, Cemile Biçer. Examination of fasting and postprandial dynamic thiol disulfide homeostasis and oxidative stress. Int J Med Biochem . 2021; 4(3): 185-191

Corresponding Author: Ahmet Rıfat Balık, Türkiye
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