Chitotriosidase might be a diagnostic marker in patients with hashimoto thyroiditis
Hatice Özışık1, Aslı Suner2, Öznur Çopur3, Eser Yıldırım Sözmen3, Banu Şarer Yürekli1, Şevki Çetinkalp1, Gökhan Özgen1, Mehmet Erdoğan11Department of Endocrinology and Metabolism, İzmir, Turkey2Department of Biostatistics and Medical Informatics, İzmir, Turkey
3Department of Medical Biochemistry, İzmir, Turkey
INTRODUCTION: Hashimoto’s thyroiditis (HT) is the most common organ-specific autoimmune disease. The aim of our study was to investigate a possible role of chitotriosidase (Chito) activity as a potential marker of inflammation in diagnosis of patients with HT. In addition, we aimed to determine the levels of inflammation markers such as YKL-40, high sensitivity C-reactive protein (hsCRP) and oxidative stress (OS) parameters such as thiobarbituric acid reactive substances (TBARS), catalase (CAT), superoxide dismutase (SOD), GSH-Px to evaluate an association between those inflammatory and OS markers in patients with HT
METHODS: 42 patients with HT and 28 control cases were studied. All cases were euthyroid. Chito activity was measured fluorometrically based on Hollak et al’s. method. hsCRP and YKL-40 levels were measured using ELISA. TBARS, SOD, and CAT activities were determined in hemolyzates. GSH-Px activity was determined by a colorimetric assay
RESULTS: Higher Chito concentrations were observed in patients with HT compared to the control group (p=0.002). YKL-40 levels were detected higher in HT but it was not statistically significant (p=0.810). Chito levels were positively correlated with age and negatively correlated with SOD (r=0.360, p=0.021; r=-0.368, p=0.018). YKL-40 levels were positively correlated with FT3 and SOD in HT (r=0.324, p=0.037; r=0.312, p=0.044) and negatively correlated with age (r=−0.463, p=0.002). SOD levels were negatively correlated with age and positively correlated with YKL-40 (r=−0.371, p=0.016, r=0.312, p=0.044).
DISCUSSION AND CONCLUSION: We revealed that Chito levels were higher among euthyroid HT patients. This result may point out that Chito levels could be used as a potential marker of inflammation in HT.
Manuscript Language: English