ISSN  2587-2362  |  E-ISSN  2618-642X
Exploratory role of serum FGF-8 as a marker of bone metastasis in tumor progression [Int J Med Biochem ]
Int J Med Biochem . 2024; 7(1): 33-40 | DOI: 10.14744/ijmb.2023.36002

Exploratory role of serum FGF-8 as a marker of bone metastasis in tumor progression

Gurpreet Singh Gill1, Meenakshi Kaushik1, Simmi Kharb1, Gitanjali Goyal2, Uttam Malik3
1Department of Biochemistry, Pt. B.D. Sharma Post Graduate Institute of Medical Sciences, Rohtak, India
2Department of Biochemistry, All India Institute of Medical Sciences, Bathinda, India
3Department of Biochemistry, Dr. Radhakrishan Government Medical College, Hamirpur, India

INTRODUCTION: FGF-8, a member of the FGF family, plays a crucial role in cellular processes and has been implicated in cancer progression. The study aims to comprehend FGF-8's involvement in bone metastasis, emphasizing its potential as a diagnostic marker and focusing on its association with Bone-Alkaline Phosphatase (B-ALP) and other biochemical parameters.
METHODS: The case-control study spans 12 months, involving 60 participants, including 30 with secondary bone metastases and an equal number without metastasis. FGF-8 levels were quantified using ELISA, and B-ALP, serum ALP, and various biochemical parameters were assessed. The study employed standardized procedures to minimize bias, including matching cases and controls, and obtaining ethical approval.
RESULTS: In patients with bone metastasis, serum ALP levels, particularly B-ALP, were significantly higher. The metastatic group exhibited elevated FGF-8 concentrations, showcasing a positive correlation with B-ALP and serum calcium levels. The study successfully differentiated ALP isoenzymes through heat inactivation and L-phenylalanine inhibition. Additionally, serum calcium levels were markedly elevated in the metastatic group.
DISCUSSION AND CONCLUSION: The findings suggest that FGF-8 is a potential diagnostic marker for bone metastasis, particularly in breast and prostate cancers. Elevated FGF-8 levels correlate with increased B-ALP and serum calcium, indicating its role in osteoblastic differentiation in metastasis. The study proposes the utility of ELISA-based kits for FGF-8 in serum as a practical and efficient method for assessing bone tumor progression.

Keywords: B-ALP, bone metastasis, diagnostic marker, FGF-8, osteogenesis

Gurpreet Singh Gill, Meenakshi Kaushik, Simmi Kharb, Gitanjali Goyal, Uttam Malik. Exploratory role of serum FGF-8 as a marker of bone metastasis in tumor progression. Int J Med Biochem . 2024; 7(1): 33-40

Corresponding Author: Meenakshi Kaushik, India
Manuscript Language: English
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