Examination of amino acid profile in patients with chronic renal failure

INTRODUCTION: This study aims to analyze the plasma free amino acid profiles pre and post dialysis in patients with chronic kidney failure (CRF), and to evaluate their potential utility in diagnosis and treatment by comparing them with profiles from a healthy control group.
METHODS: Plasma samples were collected from 46 healthy control and 46 patients diagnosed with CRF who applied to Şanlıurfa Harran University Medical Faculty Dialysis Department. Plasma free amino acid profiles were analyzed with LC-MS/MS.
RESULTS: Mean values of alanine, arginine, aspartic acid, citrulline, histidine, methionine, tyrosine, hydroxyproline, glycine, leucine, isoleucine, lysine, ornithine, phenylalanine, proline, serine glutamic acid, glutamine, valine, taurine, alloisoleucine, alphaaminoadipic acid, anserine, gammaaminobutyric acid, 1- methylhistidine, 3-methylhistidine, 5-hy-droxytryptophan levels in CRF patients exhibited higher levels compared to the control group. Phosphoethanolamine, cystine, alphaaminobutyric acid, betaaminoisobutyric acid and tryptophan were found to be lower in CRF patients than control group. When post-dialysis compared to pre-dialysis; there was an increase in citrulline, histidine, alanine, arginine, aspartic acid, glutamic acid, glycine, cystine, isoleucine, proline, phosphoethanolamine, taurine, alloisoleucin, alphaaminoadipic acid, ancerine, alphaaminobutyric acid, betaaminoisobutyric acid, beta alanin, 1-methylhistidine, 5-hydroxytryptophan levels; there was a decrease was observed in glutamine, leucine, lysine, ornithine, phenylalanine, serine, valine, asparagine, methionine, tryptophan, tyrosine, hydroxyproline, gammaaminobutyric acid, 3-methylhistidine levels. Citrulline, glycine, anserine, alphaaminobutyric acid, gammaaminobutyric acid, phosphoethanolamine and taurine levels were found to be significant in the Paired samples test, which was used to test the significance of the difference between the arithmetic means of the groups (p<0.05).
DISCUSSION AND CONCLUSION: More studies were needed to understand the role of amino acids in CRF.

Keywords: Amino acid, chronic kidney disease, LC/MS, metabolic