ISSN  2587-2362  |  E-ISSN  2618-642X
International Journal of Medical Biochemistry A histological and biochemical study of cumulus cells and the oocyte microenviroment in in vitro fertilization patients [Int J Med Biochem ]
Int J Med Biochem . 2021; 4(1): 42-49 | DOI: 10.14744/ijmb.2020.83007

A histological and biochemical study of cumulus cells and the oocyte microenviroment in in vitro fertilization patients

Nurhan Erkaya1, Tuba Demirci2, Özlem Özgul Abuc3, Mesut Halıcı4, Kamber Kasalı5
1Department of Histology and Embryology, Aksaray University Faculty of Medicine, Aksaray, Turkey
2Department of Histology and Embryology, Ataturk University Faculty of Medicine, Erzurum, Turkey
3Department of Histology and Embryology, Erzincan Binali Yildirim University Faculty of Medicine, Erzincan, Turkey
4Department of Biochemistry, Ataturk University Faculty of Veterinary Medicine, Erzurum, Turkey
5Department of Biostatistics, Ataturk University Faculty of Medicine, Erzurum, Turkey

INTRODUCTION: The aim of this study was to investigate the effect of chemical changes in the follicular fluid and histological changes in the cumulus cells of the oocyte microenvironment on the number of oocytes in infertile patients.
METHODS: A total of 50 female patients aged 18-35 who presented at the Atatürk University Research Hospital Infertility Clinic and for infertility treatment were included. The patients were divided into 3 groups: Patients with fewer than 5 oocytes were classified as Group 1, patients with 5-20 oocytes comprised Group 2, and Group 3 was made up of patients with >20 oocytes. During the oocyte collection process, follicular fluid was aspirated from the follicles and the cumulus cells were collected. The follicular fluid was stored at -80°C for use in biochemical analysis of malondialdehyde (MDA), total antioxidant status (TAS), total oxidant status (TOS), superoxide dismutase (SOD), glutathione (GSH). Immunohistochemical staining was performed to examine caspase-3 and mechanistic target of rapamycin (mTOR) immunoreactivity at the stereological level.
RESULTS: The MDA level and total oxidant capacity (TOC) in the follicular fluid were higher in Group 1 patients than in the other 2 groups, while the SOD was lower (p<0.05). In Group 2 patients, the MDA level and TOS were higher than those of Group 3, while the SOD level was lower (p<0.05). The total antioxidant capacity (TAC) and GSH levels did not vary significantly according to the number of oocytes (p<0.05). Immunohistochemical staining showed that mTOR and caspase-3 immunoreactivity were more intense in Group 1 than in the other groups.
DISCUSSION AND CONCLUSION: The increase in mTOR expression may activate the caspase-3 pathway, which could lead to oxidative stress. The mTOR pathway may affect the oocyte count.

Keywords: Caspase-3, cumulus cell, follicular fluid, in vitro fertilization, mTOR

Nurhan Erkaya, Tuba Demirci, Özlem Özgul Abuc, Mesut Halıcı, Kamber Kasalı. A histological and biochemical study of cumulus cells and the oocyte microenviroment in in vitro fertilization patients. Int J Med Biochem . 2021; 4(1): 42-49

Corresponding Author: Nurhan Erkaya, Türkiye
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