ISSN  2587-2362  |  E-ISSN  2618-642X
International Journal of Medical Biochemistry The effect of hemoglobin variants on high-performance liquid chromatography measurements of glycated hemoglobin [Int J Med Biochem ]
Int J Med Biochem . 2021; 4(1): 25-28 | DOI: 10.14744/ijmb.2020.91885

The effect of hemoglobin variants on high-performance liquid chromatography measurements of glycated hemoglobin

Gönül Şeyda Seydel1, Figen Güzelgül2
1Department of Health Care Services, Nigde Omer Halisdemir University, Nigde, Turkey
2Department of Biochemistry, Tokat Gaziosmanpasa University Faculty of Pharmacy, Tokat, Turkey

INTRODUCTION: Glycated hemoglobin (HbA1c) is routinely utilized to monitor long-term glycemic control. The presence of hemoglobin (Hb) variants may lead to a false HbA1c measurement. This study was an investigation of the effects of both common and rare Hb variants on the level of HbA1c measured with high-performance liquid chromatography (HPLC).
METHODS: The HbA1c level of a total of 391 patients without Hb variants (HbAA, n=44) and with Hb variants (HbAS, HbSS, HbSS(A), HbSS(F), HbAD, HbAE, HbAF, HbD-Iran/D-Iran, HbD-Los Angeles/A, HbE-Saskatoon/E-Saskatoon, HbEE, HbG-Coushatta/A, HbOArab/OArab, HbSE, and Hb Stanleyville II, n=347) was measured using an HPLC analyzer.
RESULTS: The HbA1c level of all of the Hb variants but HbStanleyville II and HbG-Coushatta/A was extremely low. However, when the Hb variants were considered as a single group, a statistically significant difference was seen in comparison with the group that had no Hb variants (p<0.001).
DISCUSSION AND CONCLUSION: It was determined that the measurement of HbA1c can be adversely influenced by the presence of some Hb variants. Hemoglobin variants should be investigated when the HbA1c level is incompatible with blood glucose.

Keywords: HbA1c, hemoglobin variants, high-performance liquid chromatography

Gönül Şeyda Seydel, Figen Güzelgül. The effect of hemoglobin variants on high-performance liquid chromatography measurements of glycated hemoglobin. Int J Med Biochem . 2021; 4(1): 25-28

Corresponding Author: Gönül Şeyda Seydel, Türkiye
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