ISSN  2587-2362  |  E-ISSN  2618-642X
International Journal of Medical Biochemistry Does Dietary Restriction of Amino Acids Other Than Methionine Have Any Effects on Peroxide and Superoxide Production Rates, Oxidative Protein and DNA Damage in the Liver and Heart Mitochondria of Aging Rats? [Int J Med Biochem ]
Int J Med Biochem . Ahead of Print: IJMB-29981 | DOI: 10.14744/ijmb.2021.29981

Does Dietary Restriction of Amino Acids Other Than Methionine Have Any Effects on Peroxide and Superoxide Production Rates, Oxidative Protein and DNA Damage in the Liver and Heart Mitochondria of Aging Rats?

Sevda Tanrıkulu Küçük1, Evin Ademoğlu2
1Department of Biochemistry, Medical Faculty, Demiroğlu Bilim University, İstanbul, Turkey
2Department of Biochemistry, Istanbul Faculty of Medicine, Istanbul University, İstanbul, Turkey

INTRODUCTION: The objective of this study was to evaluate the long-term effects of amino acids other than methionine in mitotic and postmitotic tissues of rats by measuring mitochondrial peroxide and superoxide production, oxidative protein and DNA damage in the liver and heart of 16-month-old rats which fed either with normal diet (ND) or protein restricted-except methionine diet (PREMD).
METHODS: 4- and 12-month-old rats were fed with either ND or PREMD for 4 months. Mitochondrial peroxide and superoxide production rates, the protein carbonyl and mtDNA 8-OHdG levels of liver and heart mitochondria were measured.
RESULTS: Mitochondrial peroxide and superoxide production rates of the liver and heart mitochondria in 16-month-old rats fed either ND or PREMD did not show any significant difference. Neither feeding with PREMD nor ND has any significant effect on protein carbonyl levels between the liver and heart mitochondria of rats at any age. In 16-month-old rats, mtDNA 8-OHdG levels were significantly higher in heart than the liver regardless of the diet.
DISCUSSION AND CONCLUSION: Mitochondrial ROS production, oxidative protein and DNA damages increase both in mitotic and postmitotic tissues with age however the increment is more prominent in heart than liver. Long term feeding with PREMD could not decelerate oxidative damage during aging neither in the heart nor in the liver.

Keywords: Mitotic tissue, postmitotic tissue, protein carbonyl, superoxide production, peroxide production, protein restriction



Corresponding Author: Evin Ademoğlu, Türkiye
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